Abstract. In this paper, we present a mechanism to capture and reestablish the state of Java threads. We achieve this by extracting a thread's execution state from the application code that is executing in this thread. This thread serialization mechanism is implemented by instrumenting the original application code through a byte code transformer, without modifying the Java Virtual Machine. We describe this thread serialization technique in the context of middleware support for mobile agent technology. We present a simple execution model for agents that guarantees correct thread migration semantics when moving an agent to another location. Our thread serialization mechanism is however generally applicable to any Java program.